dc.contributor.author |
NYAKUNDI, W.O. |
|
dc.contributor.author |
MAGOMA, G. |
|
dc.contributor.author |
OCHORA, J. |
|
dc.contributor.author |
NYENDE, A.B. |
|
dc.date.accessioned |
2012-09-25T12:45:51Z |
|
dc.date.accessioned |
2013-07-19T07:49:38Z |
|
dc.date.available |
2012-09-25T12:45:51Z |
|
dc.date.available |
2013-07-19T07:49:38Z |
|
dc.date.issued |
2011-05-01 |
|
dc.identifier.uri |
http://trisanita.org/jates/atespaper2011/ates11v1n2y2011.pdf |
|
dc.identifier.uri |
http://hdl.handle.net/123456789/1556 |
|
dc.identifier.uri |
http://hdl.handle.net/123456789/948 |
|
dc.description |
This work is licensed under the Creative Commons Attribution 3.0 Unported License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
en_US |
dc.description.abstract |
The aim was to investigate the potential for biodegradation of methomyl and
diazinon (pesticides) by white rot fungi through enrichment and isolation of methomyl
and diazinon biodegraders from horticultural soils. Five white rot fungal isolates labeled
WR1, WR2, WR4, WR9 and WR15 were cultured in a medium containing methomyl
and diazinon pesticides as the only carbon source and incubated at 280C and
monitored for biodegradation at intervals of 10 days for a period of 100 days. Gilson
HPLC system was used for the separation and quantification of the pesticides. The
pesticide methomyl was detected at 4.9 minutes while the methomyl metabolite was
detected at 4.1 minutes. Diazinon was detected at 11 minutes while the diazinon
metabolites; diazoxon and oxypyrimidine were detected at 2.3 and 2.6 minutes. The
rate of biodegradation of pesticides was much higher for mixtures of fungi
(approximately 50 days) compared to individual isolates (60- 100 days) for diazinon
while methomyl was 22-25 days. This shows that fungal mixtures in soil fasten the rate
of biodegradation of pollutants compared to individual isolates. The isolates are good
biodegraders due to their ability to degrade methomyl or diazinon hence making the
isolates good agents for bioremediation. The authors recommend more studies to be
done in the fields before their potential application by farmers. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
Directory of of Open access journals |
en_US |
dc.subject |
Biodegradation |
en_US |
dc.subject |
HPLC |
en_US |
dc.subject |
Metabolite and White rot fungi isolates |
en_US |
dc.title |
Biodegradation of diazinon and methomyl pesticides by white rot fungi from selected horticultural farms in Rift Valley and Central Kenya |
en_US |
dc.type |
Article |
en_US |