Molecular screening for Plasmodium falciparum resistance markers for artemisinins in Mbita, Kenya

Show simple item record Wangai, Laura N. Geoffrey, Muriira Omar, Sabah Magoma, Gabriel Kimani, Francis T. Mwangi, Jonathan M. Burugu, Marion W. Maina, Naomi 2012-09-27T11:07:34Z 2013-07-19T07:46:37Z 2012-09-27T11:07:34Z 2013-07-19T07:46:37Z 2011
dc.identifier.issn 1595-689X
dc.description.abstract Artemisinins-based combination therapies (ACTs) are being recommended against uncomplicated malaria in endemic areas of Africa. However, in these areas data on their long term usefulness is limited. It has been demonstrated that ACTs resistance may be due to single nucleotide polymorphisms (SNPs) in the chemotherapeutic target, the SERCA-type ATPase protein (PfATPase6). This study analyzed PfATPase6 mutations in asymptomatic infections from samples collected from Mbita, a malaria endemic region in Kenya. Mutations in A623E and S769N residues were screened with gene specific primers followed by sequencing. The study demonstrates that there is no mutation in Mbita, Kenya because neither A623E nor S769N PfATPase6 mutations were detected. Resurgence of infections in this area could be due to re-infections and not drug failure. The study recommends that other sites be assessed for PfATPase 6 mutations to verify the long-term usefulness of ACT and monitor any emergency of resistance. en_US
dc.description.sponsorship Jomo Kenyatta University of Agriculture and Technology (JKUAT) en_US
dc.language.iso en en_US
dc.publisher AJOL en_US
dc.relation.ispartofseries * AJOL * Journals * Log In * Register * Journal Home * Advanced Search * Current Issue * Archives Username Password Remember me or Register Find Journals on AJOL HOW TO USE AJOL... * for Researchers * for Librarians * for Authors OTHER RESOURCES... * for Researchers * for Journals FAQ's AJOL jobs More about AJOL AJOL'S Partners Terms and Conditions of Use African Journal of Clinical and Experimental Microbiology 2011;Vol 12, No 3
dc.subject ACT (Artemisinins-based Combination Therapy) en_US
dc.subject Molecular en_US
dc.subject Mutations en_US
dc.subject PfATPase 6 en_US
dc.title Molecular screening for Plasmodium falciparum resistance markers for artemisinins in Mbita, Kenya en_US
dc.type Article en_US

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  • College of Health Sciences (COHES) [770]
    Medical Laboratory; Agriculture & environmental Biotecthology; Biochemistry; Molecular Medicine, Applied Epidemiology; Medicinal PhytochemistryPublic Health;

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