Abstract:
The major objective of seedless grape breeding prog
ram is production of grapes with
high quality. Breeding of seedless grapes is diffic
ult because the embryos are aborted
after fertilization. Embryo rescue technique is uti
lized to obtain plants from crosses
between seedless cultivars. In the present study, t
he effect of paternal genotypes and
different IAA concentrations in presence of BA appl
ications were investigated on embryo
rescue in Askari cultivar in randomized complete bl
ock design with three replications.
Sprays with BA were performed two times at 18 E-L a
nd 27 E-L stages. Hybrid embryos
were produced from the cross between Askari cultiva
r as a female parent and Ruby
Seedless, Bidane Sefid, and Bidane Ghermez as male
parents. Forty days after pollination
(at 29 E-L stage), ovules were cultured on NN mediu
m with three different concentrations
of IAA (1, 1.75, 3 mg L
-1
). The results showed that male genotypes and diffe
rent
concentrations of IAA on the media had a significan
t effect on embryo germination. BA
treatment did not affect the embryo germination sol
ely. But, the effect of BA and different
concentrations of IAA was significant on embryo ger
mination. The best concentration of
IAA with BA treatment was 1 mg L
-1
and without BA was 3 mg L
-1
. The highest embryo
germination rate was observed in 'Askari×Ruby Seedl
ess' cross that was pretreated with
BA and cultured on medium with 1 mg L
-1
IAA concentration.
Keywords
: Benzyladenine, Culture medium, Embryo rescue, Gra
pevine, Seedlessness.
