Development of an SNP Marker for Sugar Beet Resistance/Susceptible Genotyping to Root-Knot Nematode

Abstract

Linked and/or gene-based molecular markers have bee n used widely in marker- assisted selection (MAS) to differentiate resistant and susceptible genotypes. Resistance to Meloidogyne spp. in Beta vulgaris L. is mediated by a single dominant gene ( R6m-1 ). Using allele-specific primers (ASPs), an SNP marker harbo ring a single nucleotide polymorphism (A/G), linked to the resistance gene w as developed to differentiate resistant genotypes. The differentiation among the resistant and susceptible genotypes was elucidated in the polymorphic bands of 555, 478 and 124 bp in size, using PCR amplification. The genotyping data using the SNP ma rker was firmly associated with the bioassay evaluation in the greenhouse for 100 sugar beet genotypes. This data indicated that the present robust marker allowed reliable, se nsitive, faster, and cheaper large scale screening of B. vulgaris genotypes for nematode resistance breeding program s. Keywords: Beta vulgaris , Meloidogyne spp., Selection, SNP marker.