Abstract:
Linked and/or gene-based molecular markers have bee
n used widely in marker-
assisted selection (MAS) to differentiate resistant
and susceptible genotypes. Resistance to
Meloidogyne
spp. in
Beta vulgaris
L. is mediated by a single dominant gene (
R6m-1
). Using
allele-specific primers (ASPs), an SNP marker harbo
ring a single nucleotide
polymorphism (A/G), linked to the resistance gene w
as developed to differentiate resistant
genotypes. The differentiation among the resistant
and susceptible genotypes was
elucidated in the polymorphic bands of 555, 478 and
124 bp in size, using PCR
amplification. The genotyping data using the SNP ma
rker was firmly associated with the
bioassay evaluation in the greenhouse for 100 sugar
beet genotypes. This data indicated
that the present robust marker allowed reliable, se
nsitive, faster, and cheaper large scale
screening of
B. vulgaris
genotypes for nematode resistance breeding program
s.
Keywords:
Beta vulgaris
,
Meloidogyne
spp., Selection, SNP marker.
