Detection of PVY, PVX, PVS, PVA, and PLRV on Differ ent Potato Varieties in Turkey Using DAS-ELISA

Abstract

This research was performed in order to study and d iagnose Potato virus Y , Potyvirus (PVY), Potato virus X , Potexvirus (PVX), Potato virus S , Carlavirus (PVS), Potato virus A , Potyvirus (PVA) and Potato leafroll Luteovirus (PLRV) on tubers and leaves of different potato varieties, namely, Solea, Safran, Floris, Pr oventa, Milva, Universa, Lady olympia, Vangogh, and Marabel grown in Afyon region of Turke y. For this purpose, potato tubers from different varieties were obtained from Afyon r egion producers during 2009-2010 and they were planted in the trial plots in Isparta region of Turkey. One hundred sixty nine samples were taken from the leaves showing vir us symptoms in the vegetation period and 109 samples were taken from the tubers of suspi cious plants in the harvest period. Total of 278 samples were tested by using double an tibody sandwich- enzyme linked immunosorbent assay (DAS-ELISA) method. The DAS-ELI SA analysis revealed that both tubers and leaves were infected with PVY, PVX, PVS, PVA and PLRV. It was determined that 87.45% (244 samples) of the tested samples were infected with one or more viruses and 12.54% (34 samples) of them gave n egative reaction with DAS-ELISA. Regarding the prevalence of viruses among the potat o varieties in this study, it was found that all samples belonging to Safrane and Milva var ieties were infected with one or more viruses. Besides, other potato varieties showed dif ferent rates of virus infection. In the mechanical inoculation tests, serious stunting, systemic chlorosis and leaf defor mation symptoms were observed on N. glutinosa , while symptoms such as mottling, leaf distortion, chlorotic and necrotic local lesions we re observed on the leaves of other test plants. Keywords: Detection , Solanum tuberosum , Virus diseases.