Antimicrobial and Anti-oxidative Effects of Methano lic Extract of Dorema aucheri Boiss.

Abstract

Dorema aucheri Boiss. (Apiaceae) is an endemic plant growing in I ran. This plant is used as food and its extracts are recommended for t he treatment of a wide range of diseases. To conduct this study, leaf, stem, and fl ower of D. aucheri were collected from near Yasouje, Iran. Antioxidant and antimicrobial effects of methanol extracts were measured. Further, total phenolic, flavonoid, anthocyanin, carotenoid, soluble sugar, gallic acid, chlorogenic acid, caffeic acid, and p -coumaric acid contents of plant methanol extracts were also determined. The results showed t hat total phenolic, anthocyanin, and soluble sugar in the stem of D. aucheri were 22.72 mg GAE g -1 dW, 19.33 mg g -1 dW, and 6.45 mg g -1 dW respectively, greater than those of the other sa mples tested. Also, phenolic acids were identified by RP-HPLC and chlorogenic acid was the predominant phenolic compound in the samples. The highest amount of flav onoid (1.95 mg QE g -1 dW) was observed in the flower. All of the different extrac ts exhibited a good antioxidant activity based on inhibition of fatty acid oxidation assay. The maximal inhibition was observed in the leaf (48.52%) and flower (54.24%) of D. aucheri . Ferric reducing antioxidant power (FRAP) was also high in the stem and flower. In add ition, the results showed that leaf, stem, and flower extracts had inhibitory activity a gainst four bacteria tested. The highest antimicrobial activity was obtained with flower ext ract. These results suggest that the methanolic extracts from different parts of D. aucheri are a valuable source of effective compounds. The antioxidant and antimicrobial activi ty of extracts was correlated with p - coumaric and caffeic acid content. Keywords: Antioxidant, Antimicrobial activity, Dorema aucheri , Phenolic compounds.