Abstract:
Dorema aucheri
Boiss. (Apiaceae) is an endemic plant growing in I
ran. This plant is
used as food and its extracts are recommended for t
he treatment of a wide range of
diseases. To conduct this study, leaf, stem, and fl
ower of
D. aucheri
were collected from
near Yasouje, Iran. Antioxidant and antimicrobial
effects of methanol extracts were
measured. Further, total phenolic,
flavonoid, anthocyanin, carotenoid, soluble sugar,
gallic acid, chlorogenic acid,
caffeic acid, and
p
-coumaric acid contents
of plant methanol
extracts were also determined. The results showed t
hat total phenolic, anthocyanin, and
soluble sugar in the stem of
D. aucheri
were 22.72 mg GAE g
-1
dW, 19.33 mg g
-1
dW, and
6.45 mg g
-1
dW respectively, greater than those of the other sa
mples tested. Also, phenolic
acids were identified by RP-HPLC
and chlorogenic acid was the predominant phenolic
compound in the samples. The highest amount of flav
onoid (1.95 mg QE g
-1
dW) was
observed in the flower. All of the different extrac
ts exhibited a good antioxidant activity
based on inhibition of fatty acid oxidation assay.
The
maximal inhibition was observed in
the leaf (48.52%) and flower (54.24%) of
D. aucheri
. Ferric reducing antioxidant power
(FRAP) was also high in the stem and flower. In add
ition, the results showed that leaf,
stem, and flower extracts had inhibitory activity a
gainst four bacteria tested. The highest
antimicrobial activity was obtained with flower ext
ract. These results suggest that the
methanolic extracts from different parts of
D. aucheri
are a valuable source of effective
compounds. The antioxidant and antimicrobial activi
ty of extracts was correlated with
p
-
coumaric and caffeic acid content.
Keywords:
Antioxidant, Antimicrobial activity, Dorema aucheri
,
Phenolic compounds.
