Micropropagation of Astragalus adscendens : A Source of Gaz-angabin Manna in Iran (Persian Manna)

Abstract

The aim of this study was to investigate the in vitro proliferation of Astragalus adscendens . Explants were taken from hypocotyl and cotyledon and were cultured on the basic medium of Murashige and Skoog (MS) complement ed with various plant growth regulators, (NAA, BAP, KIN, ZEA), to induce direct shoot regeneration. Callus induction was significantly affected by different concentrati ons of PGRs. Callus formation was observed from hypocotyl explants, but they were not induced to adventitious shoot regeneration and most of them were turned into brow n. Therefore, rapid multiplication, performed using shoot apical buds, and obtained fro m 15-day old sterile seedlings. Apical buds were cultured on MS medium containing various levels of BAP, KIN and ZEA (0.5, 1.0, 2.0 and 4.0 mg L -1 ) alone or in combination with 0.5 mg L -1 NAA. The highest number of shoot regenerants (8.5/explants) and leaves (22. 4/explants) obtained on MS medium with 4 mg L -1 BAP . The highest root induction (100%) was obtained fro m MS media supplemented with 0.3 mg L -1 NAA. Rooted plantlets were successfully acclimatize d in pots with 1:1:1 mixture of soil, peat, and perlite. Keywords : Apical buds, Callus induction, Clonal propagation, Gavan-e-Gaz-a ngabin, Proliferation