Abstract:
The aim of this study was to investigate the
in vitro
proliferation of
Astragalus
adscendens
. Explants were taken from hypocotyl and cotyledon
and were cultured on the
basic medium of Murashige and Skoog (MS) complement
ed with various plant growth
regulators, (NAA, BAP, KIN, ZEA), to induce direct
shoot regeneration. Callus induction
was significantly affected by different concentrati
ons of PGRs. Callus formation was
observed
from hypocotyl explants, but they were not induced
to adventitious shoot
regeneration and most of them were turned into brow
n. Therefore, rapid multiplication,
performed using shoot apical buds, and obtained fro
m 15-day old sterile seedlings. Apical
buds were cultured on MS medium containing various
levels of BAP, KIN and ZEA (0.5,
1.0, 2.0 and 4.0 mg L
-1
) alone or in combination with 0.5 mg L
-1
NAA. The highest number
of shoot regenerants (8.5/explants) and leaves (22.
4/explants) obtained on MS medium
with 4 mg L
-1
BAP
.
The highest root induction (100%) was obtained fro
m MS media
supplemented with 0.3 mg L
-1
NAA. Rooted plantlets were successfully acclimatize
d in
pots with 1:1:1 mixture of soil, peat, and perlite.
Keywords
: Apical buds,
Callus induction, Clonal propagation, Gavan-e-Gaz-a
ngabin, Proliferation
