Heat-shock Protein Synthesis Study by Micro-chip

Abstract

Keeping constant all agronomic factors except tempe rature is a way to follow Heat Shock Proteins (HSPs) expression. Three different s unflower hybrids derived from the cross between an inbred line, Cms HA 89, and three different restorer inbred lines, with resistance, susceptible, and normal reactions to dr ought, were produced. In order to investigate the impact of temperature on protein ac cumulation during achene filling phase, they were cultivated in two different geogra phical areas: Karaj in Iran and Udine in Italy. Total protein content and structural poly peptide fractions of Seed Storage Proteins (SSPs) were determined. The analysis of HS Ps was carried out by means of lab- on-chip capillary electrophoresis. It was revealed that protein accumulation in achene occurs at a greater rate during the achene filling phase, i.e. approximately 9-25 days after pollination, in all examined hybrids. Besides, the presence of a polypeptide band of 17.7 kDa supposed to be of small Heat Shock Proteins (sH SPs) family was recorded in all three hybrids grown up in hot and arid environment, Karaj , which implied independent sHSPs expression from the paternal restorer line. Keywords : Hybrid sunflower, Lab-on-chip capillary electrophor esis, Paternal restorer line, Small Heat Shock Protein (sHSPs).