Abstract:
Keeping constant all agronomic factors except tempe
rature is a way to follow Heat
Shock Proteins (HSPs) expression. Three different s
unflower hybrids derived from the
cross between an inbred line, Cms HA 89, and three
different restorer inbred lines, with
resistance, susceptible, and normal reactions to dr
ought, were produced. In order to
investigate the impact of temperature on protein ac
cumulation during achene filling
phase, they were cultivated in two different geogra
phical areas: Karaj in Iran and Udine
in Italy. Total protein content and structural poly
peptide fractions of Seed Storage
Proteins (SSPs) were determined. The analysis of HS
Ps was carried out by means of lab-
on-chip capillary electrophoresis. It was revealed
that protein accumulation in achene
occurs at a greater rate during the achene filling
phase, i.e. approximately 9-25 days after
pollination, in all examined hybrids. Besides, the
presence of a polypeptide band of 17.7
kDa supposed to be of small Heat Shock Proteins (sH
SPs) family was recorded in all three
hybrids grown up in hot and arid environment, Karaj
, which implied independent sHSPs
expression from the paternal restorer line.
Keywords
:
Hybrid sunflower, Lab-on-chip capillary electrophor
esis, Paternal restorer line,
Small Heat Shock Protein (sHSPs).
