Green Synthesis of Silver Nano-particles Using Kelussia odoratissima Mozaff. Extract and Evaluation of its Antibacterial Activi

Abstract

In this research Kelussia odoratissima Mozaff. leaf extract was used for the green synthesis of silver nanoparticles (AgNPs). At first we compared antioxidant activity of different extracts of K. odoratissima . Then solution containing silver nitrate was treat ed with the extract which showed high antioxidant acti vity. Synthesized AgNPs were evaluated by analyzing the excitation of surface pl asmon resonance. TEM analysis was also used for nanoparticle characterization. Antiba cterial activity of the solution containing AgNPs was measured by microdilution test . Common food contaminant bacteria such as gram-positive ( Staphylococcus aureus , Bacillus cereus , Listeria monocytogenes ) and gram-negative ( Escherichia coli O157: H7, Salmonella enterica and Pseudomonas aeruginosa ) were used for the evaluation. The aqueous extract s howed the highest antioxidant activity and the solution was u sed for the green synthesis of AgNPs. The particle diameters were calculated to be 20-40n m with -17 to -19.9 mV zeta potential. The TEM micrographs showed that the AgNPs are nearl y spherical in shape and highly monodispersed. MIC of the AgNPs against gram-positi ve and gram-negative bacteria was between 0.012-0.025 and 0.006-0.012 mg/ml respectiv ely. Keywords: Kelussia odoratissima, Green synthesis, Silver nanoparticles , Antibacterial activity, Electron microsco