Abstract:
In this research
Kelussia odoratissima
Mozaff. leaf extract was used for the green
synthesis of silver nanoparticles (AgNPs). At first
we compared antioxidant activity of
different extracts of
K. odoratissima
. Then solution containing silver nitrate was treat
ed
with the extract which showed high antioxidant acti
vity. Synthesized AgNPs were
evaluated by analyzing the excitation of surface pl
asmon resonance. TEM analysis was
also used for nanoparticle characterization. Antiba
cterial activity of the solution
containing AgNPs was measured by microdilution test
. Common food contaminant
bacteria such as gram-positive (
Staphylococcus aureus
,
Bacillus cereus
,
Listeria
monocytogenes
) and gram-negative (
Escherichia
coli
O157: H7,
Salmonella
enterica
and
Pseudomonas
aeruginosa
)
were used for the evaluation. The aqueous extract s
howed the
highest antioxidant activity and the solution was u
sed for the green synthesis of AgNPs.
The particle diameters were calculated to be 20-40n
m with -17 to -19.9 mV zeta potential.
The TEM micrographs showed that the AgNPs are nearl
y spherical in shape and highly
monodispersed. MIC of the AgNPs against gram-positi
ve and gram-negative bacteria was
between 0.012-0.025 and 0.006-0.012 mg/ml respectiv
ely.
Keywords:
Kelussia odoratissima,
Green synthesis, Silver nanoparticles
,
Antibacterial
activity, Electron microsco
