Isolation and characterization of Streptomyces species with antifungal activity from selected national parks in Kenya

Show simple item record Nonoh, James Oluoch Lwande, Wilber Masiga, Daniel Herrmann, Rafi Presnail, James K. Schepers, Eric Okech, Matilda Angela Bagine, Richard Mungai, Paul Nyende, Aggrey Bernard Boga, Hamadi Iddi 2012-09-25T11:14:04Z 2013-07-19T07:49:37Z 2012-09-25T11:14:04Z 2013-07-19T07:49:37Z 2010-05
dc.identifier.issn 1996-0808
dc.description Abstract of a research paper. en_US
dc.description.abstract Microorganisms and their natural products are potentially important for the biological control of crop diseases without detrimental effects to the environment. In this study, acetonitrile-methanol extracts of 361 actinobacterial isolates obtained from Aberdares, Arabuko Sokoke, Lake Bogoria, Mt Kenya, Kakamega, Ruma, Shimba Hills and Imenti forest national parks in Kenya were screened for antagonism against Fusarium oxysporum, Fusarium spp and Colletotrichum kahawae, which are important crop pathogens. Twenty-three isolates showed antagonistic activity to one or all of the test fungi. Five isolates that were antagonistic against all test fungi were investigated further and were also found to have antibacterial activity against Staphylococcus aureus and Escherichia coli. Morphological and physiological studies show that the isolates belong to streptomycetes. Phylogenetic analysis of amplified actinobacterial 16S rRNA gene confirmed that all the five antagonistic isolates formed close phylogenetic clusters with known members of Streptomyces species with a (97 - 100%) sequence identity. The results suggest that protected areas may be ideal habitats for isolation of antagonistic actinobacterial species which may have the potential for beneficial application in biological control of fungal pathogens. However, further investigation by characterization of the antifungal and antibacterial compounds produced will be necessary. en_US
dc.description.sponsorship International Center of Insect Physiology and Ecology (ICIPE), Botany Department, Jomo Kenyatta University of Agriculture and Technology (JKUAT), Kenya Wildlife Service (KWS), Pioneer, A DuPont Company, Crop Genetics Research and Development. en_US
dc.language.iso en en_US
dc.publisher Academic Journals en_US
dc.relation.ispartofseries African Journal of Microbiology Research;Vol. 4 (9)
dc.subject Protected areas en_US
dc.subject soil streptomyces en_US
dc.subject bio-prospecting en_US
dc.subject antimicrobial en_US
dc.subject phytopathogens en_US
dc.title Isolation and characterization of Streptomyces species with antifungal activity from selected national parks in Kenya en_US
dc.type Article en_US

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