Abstract:
In this study, 120 accessions of Aegilops crassa collected from various geographical
areas of Iran were analyzed with respect to genome size and protein markers. A flow
cytometry survey of these accessions revealed that one hundred and thirteen of the
accessions were tetraploid and seven were hexaploid. Moreover, these accessions revealed
variations in high molecular weight glutenin subunit compositions. In most accessions,
subunits showing electrophoretic mobility similar to that of Dy12 were present. Eleven
allelic variants were observed in Glu-D1 locus with the highest (30.90%) and the lowest
allele (0.5%) frequencies in 3+12 and 2+10 variants, respectively. Among 17 bands
selected for MALDI-TOF-TOF-MS analysis only 6 bands were identified with high
probability and 11 of them had no MS/MS data. The results showed that Iranian
accessions of Ae. crassa formed an interesting source of favorable glutenin subunits that
might be very desirable in breeding programs for improving bread wheat quality.
Keywords: Aegilops crassa, Glutenin, Mass spectrometry, Protein, SDS-PAGE.
