Abstract:
ABCG2
(ATP binding cassette subfamily G member 2) gene,
located on chromosome 6
encodes the
ABCG2
protein that transports various xenobiotics, cytos
tatic drugs across
the plasma membrane as well as cholesterol into mil
k. A single nucleotide change (A/C) in
base 86 of exon 14 is capable of encoding a substit
ution of tyrosine with serine in the
ABCG2
gene and increase milk yield while decreasing milk
fat and protein
concentrations. The major aim followed in this rese
arch was to study Single Nucleotide
Polymorphisms (SNPs) of
ABCG2
gene and their association with milk production tr
aits
in Iranian Holstein bulls. Genomic DNA of 105 ified
bulls was extracted from semen
samples using highly Pure PCR template preparation
kit. Primers were designed through
Oligo software (Version 5.0) and utilized in PCR. T
hen the PCR fragments were
sequenced. The
A/C
substitution in base number 86 of exon 14 was obser
ved with 2%
frequency which affected protein percentage (P< 0.0
5). Some SNPs were detected for the
first time in intron 13, exon and intron 14 in comp
arison with sequences in the NCBI
database. A deletion mutation in base number 20 (T/
-) and a missense mutation in base
number 67 (A/G) of exon 14 that cause the substitut
ion of serine with glycine were
discovered which were significantly associated with
protein yield and fat percentage,
respectively (P< 0.05). Furthermore, significant as
sociation was observed between fat
percentage and mutations in base numbers 4,133 (T/C
) and 4,137 (T/G) of intron 13 (P<
0.05). Substitutions in base numbers 2 (T/C) and 55
(G/C) of intron 14 resulted in a
significant effect on fat yield and fat percentage
(P< 0.05).
Keywords:
ABCG2
gene, Holstein bulls
,
Milk production traits, Polymorphism.
