Abstract:
Tarkhineh is one of the traditional foods in Iran
and a rich source of probiotic
bacteria. The objective of this study was to identi
fy the probiotic bacteria isolated from
Tarkhineh using 16S rRNA gene sequencing and molecu
lar typing with repetitive
extragenic palindromic–PCR (REP-PCR). In total, 20
different bacteria were isolated
from traditional dairy products and Tarkhineh. Mole
cular identification of the isolates
was carried out by 16S rRNA gene sequencing, and DN
A sequences of isolates deposited
in GenBank. The REP-PCR reaction by REP1R-I, REP2-I
and REP1R-I+REP2-I
markers was performed for fingerprinting and charac
terization of the isolates. Un-
weighted pair group method with arithmetic mean (UP
GMA) clustering methods were
performed based on Dice similarity. The REP1R-I pri
mer grouped isolates into three, and
REP2-I and REP1R-I+REP2-I grouped all isolates into
four main clusters in
dendrograms. In all analyses, isolates of
Lactobacillus casei
,
Lactobacillus brevis,
Lactobacillus plantarum
, and
Entrococcus facium
formed separate clusters. The results of
sequencing corresponded to clustering in the dendro
gram. According to the results, REP-
PCR is an accurate technique for determining the ge
netic diversity of lactic acid bacteria
species.
Keywords
: 16S rDNA sequencing, REP-PCR fingerprinting.
