Transformation of Insect and Herbicide Resistance G enes in Cotton ( Gossypium hirsutum L.)

Show simple item record Awan, M. F. Abbas, M. A. Muzaffar, A. Ali, A. Tabassum, B. Rao, A. Q. Nasir, I. Ahmad Husnain, T. 2018-02-20T06:58:21Z 2018-02-20T06:58:21Z 2018-02-20
dc.description Paper en_US
dc.description.abstract Manipulation of different genes in crop plants to g et desirable characters has become an important tool of plant biotechnology. In the cu rrent study, cotton variety MNH-786 was modified for its characteristics to show resist ance against lepidopteran insects and herbicide by transformation of Cry1Ac+Cry2A and GTGene cloned in a different cassette under 35S Promoter. Mature embryos of cotton MNH-78 6 were injured by a sharp blade at the shoot apex and infected with the Agrobacterim tumefaciens harboring transgene constructs. Transformed cotton plants were successf ully acclimatized in pots and later the green house. Gene specific PCR and ELISA confirmed the transgene presence and its protein expression which was considerably higher in transformed plants. Overall transformation efficiency was 1.05%. All larvae of Helicoverpa armigera feeding on transgenic cotton leaves of T 0 were found dead as compared to the control ones fe eding on leaves from non-transgenic cotton. Transgenic plant s also survived a glyphosate spray dose of 1,900 ml acre -1 as compared to herbs/weeds growing along with them , which burned completely five days post glyphosate applica tion. Keywords: Bt cotton, Glyphosate, Transgenic, Weedicide resis tance. en_US
dc.language.iso en en_US
dc.publisher JKUAT en_US
dc.subject Weedicide resistance. en_US
dc.subject Transgenic en_US
dc.subject Glyphosate en_US
dc.subject Bt cotton en_US
dc.title Transformation of Insect and Herbicide Resistance G enes in Cotton ( Gossypium hirsutum L.) en_US
dc.type Working Paper en_US

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