Abstract:
Peanut plants showing mottling, yellow and necrotic
spots on leaves were collected
from peanut fields in Golestan province. Electron m
icroscopic studies revealed the
presence of flexuous filamentous particles ca. 700
nm in length, which was suggestive of a
potyvirus infection. Healthy
Nicotiana benthamiana
plants mechanically inoculated with
sap from infected peanut plants showed mottling, do
wnward leaf curling, and wrinkling
of the leaves. The virus was transmitted by
Myzus persicae
in a non-persistent manner to
healthy
N. benthamiana,
on which symptoms were observed two weeks later. R
T-PCR
using an Oligo-dT and a NIb primer set resulted in
a fragment of about 1093 bp, which
comprised the complete coat protein (CP) gene and 3
́-non-coding region. Analysis of its
CP nucleotide and amino acid sequence revealed 98-9
9% similarity and 95-99% identity
to those of
Peanut mottle virus
(PeMoV) isolated from other countries, respectivel
y. The
molecular data confirmed serological, vector transm
ission, and electron microscopic
findings on the incidence of PeMoV in Iran. Additio
nally, sequence and phylogenetic
analyses of the CP revealed clustering of Iranian P
eMoV isolate with Asian/Australian
isolates.
Keywords
:
Arachis hypogea
, Phylogenetic analysis,
Potyviridae
.
