Abstract:
The objective of this study was to explore the mic
robial community diversities and the
relationships between microbial community compositi
ons and hydrochemical factors in
nitrate contaminated groundwater of Hun River alluv
ial plain. The method of polymerase
chain reaction (PCR)-denaturing, gradient gel elect
rophoresis (DGGE) gene fingerprints
combined with canonical correspondence analysis
（
（（
（
CCA
）
））
）
were applied. The
Operational Taxonomic Units (OTUs) of all the sampl
ing sites had a certain degree of
heteroplasmy and 75% OTUs presented in less than ha
lf of the sampling sites. The un-
weighted pair group mean average (UPGMA) cluster an
alysis showed that the microbial
community similarity of all the sampling sites were
not relatively high (0.6-0.8). The
distribution of microbial community positively corr
elated with nitrate. The dominant
bacteria of the nitrate contaminated groundwater ma
inly included
Hyphomicrobium
denitrifican
sp.
, Halanaerobium praevalens
sp.
, Desulfotomaculum reducens
sp.
,
Nitrosospira multiformis
sp., among which
the Nitrosospira multiformis
sp.
and
Sulfurovum
sp. existed in all the sampling sites. CCA results
indicated that Mn
2+
and NO
3
-
were the
most relevant hydrochemical factors to regulate the
microbial composition in nitrate
contaminated groundwater of this area, and next wer
e Fe
2+
and SO
4
2-
. The results could
provide references for the bioremediation of the ni
trate contaminated groundwater of
Hun River alluvial plain
Keywords:
Bacteria, Bioremediation, PCR-denaturing.
