Protein Marker Assisted Identification of Yr9, Lr26 and Sr31 Genes in a Group of Iranian Wheat Cultivars

Abstract

The 1RS chromosome segment derived from Petkus rye carries genes for resistance to three wheat rust diseases, namely Lr26 for resistance to leaf rust (caused by Puccinia triticina ), Yr9 for resistance to stripe rust ( P.striiformis f. sp. tritici ) and Sr31 for resistance to stem rust ( P. graminis f. sp. tritici ). Since Sec-1 is tightly linked with the three rust resis- tance genes electrophoresis it is a useful method to identify and confirm the presence of three rust resistance genes in current wheat populations. SDS-PAGE was used to examine eight Iranian wheat cultivars for resistance to three rusts. The eight Iranian wheat culti- vars examined were Alvand, Darab 2, Tajan, Nicknejad, Mahdavi, Zarrin, Alamoot and Atrak. The SDS-PAGE results showed that cultivars Mahdavi and Atrak have Sec-1 bands and are therefore likely to carry the 1BL.1RS translocation and the linked genes Yr9, Lr26 and Sr31.