Abstract:
J. Agr. Sci. Tech. (2016) Vol. 18: 545-552
545
Identification of SSR Marker Linked to Leaf Rust Re
sistant
Gene
Lr24
and Marker Assisted Transfer of Leaf Rust
Resistance Genes
into Bread Wheat
B. S. Savitha
1
, S. A. Desai
1
∗
, S. S. Biradar
1
, R. Naik
1
, and H. C. Lohithaswa
2
ABSTRACT
A study was conducted to identify SSR markers linke
d to leaf rust resistance genes
Lr24
and
Lr28
and to be used for Marker-Assisted Selection (MAS)
to transfer both genes to a
widely cultivated wheat variety MP 3299 under rainf
ed condition. F2 individuals of the
cross MP 3299×NIL PBW 343 were used for generating
genotypic data employing closely
linked SCAR markers S73719 and S421570 to
Lr24
and
Lr
28, respectively, and further
subjected to bulk segregant analysis. A total of 70
SSR markers that amplify sequences on
long arm of chromosome
3D and long arm of chromosome 4A were used for
polymorphism assay between the parents MP 3299 and
NIL PBW 343. Eighteen SSRs
were polymorphic between the parents, of which 10 w
ere located on chromosome 3DL
and eight on chromosome 4AL. Three SSR markers out
of 18 polymorphic markers
differentiated two contrasting bulks and further us
ed for F2 genotyping. Finally, one SSR
marker i.e. ‘barc 71’ linked to SCAR marker SCS7371
9 at a distance of 3.36 cM based on
the per cent recombination frequency was identified
. Thus, the newly identified SSR
marker barc 71 linked to
Lr24
can serve as a useful marker in gene pyramiding in
stead of
SCAR marker SCS73719.
Keywords
: Bulked segregant analysis, Major gene, Marker-ass
isted selection, Seedling
resistance, Polymorphism
