Plant Regeneration from Cell Suspension Protoplasts of Two Iranian Japonica Rice Cultivars and Ploidy Level of Regenerated Plants

Jelodar1, N. B.; Davey2, M. R.; Cocking, E. C.

dc.contributor.author	Jelodar1, N. B.
dc.contributor.author	Davey2, M. R.
dc.contributor.author	Cocking, E. C.
dc.date.accessioned	2018-02-13T09:19:19Z
dc.date.available	2018-02-13T09:19:19Z
dc.date.issued	2018-02-13
dc.identifier.uri	http://hdl.handle.net/123456789/4077
dc.description.abstract	An efficient system has been developed for the reproducible regeneration of plants from cell suspension-derived protoplasts of the two commercial Iranian Japonica rice cultivars Tarom and Khazer. Friable embryogenic calli were used for cell suspension initiation and protoplasts were isolated from both varieties. When embedded in agarose, protoplasts failed to divide. However, sustained divisions were obtained by using nurse cells of Lolium moltiflorum. Plant regeneration was 21.1% and 10.5% for Tarom and Khazer, respectively. Somaclonal variation was observed amongst regenerated plants, 16.2% of regenerants were tetraploid, and the rest were diploid.	en_US
dc.language.iso	en	en_US
dc.publisher	AGRICUTURE - JKUAT	en_US
dc.subject	Cell suspension, Iranian Japonica rice	en_US
dc.subject	Oryza sativa,	en_US
dc.subject	Plant regeneration	en_US
dc.subject	Ploidy,	en_US
dc.title	Plant Regeneration from Cell Suspension Protoplasts of Two Iranian Japonica Rice Cultivars and Ploidy Level of Regenerated Plants	en_US
dc.type	Working Paper	en_US