Abstract:
The detection of Genetically Modified (GM) organism
s is becoming a legal necessity.
This study was carried out to detect genetically mo
dified events in soybeans imported into
Iran using simplex and multiplex PCR. Therefore, fi
ve samples of imported soybean were
obtained from Bandar Imam Khomeini customs. Modifie
d CTAB method was used to
extract DNA from soybean seeds. The result indicate
s that the modified method is suitable
for DNA extraction from soybean seeds and probably
can be used for other oilseeds.
Using specific primers for
CaMV 35S
promoter,
NOS
terminator and
epsps
gene PCR
reactions were performed. In this study soybean
lectin
gene was used as internal control.
The results revealed that soybean samples imported
from Canada and Paraguay were
genetically modified and they had
CaMV 35S
promoter,
NOS
terminator and
epsps
gene
in their genomes. The result of simplex PCR was the
same as multiplex PCR, but
multiplex PCR detected the GM soybeans very quickly
and in a cost-saving and time-
consuming way. Based on PCR analysis using GM soybe
an event-specific primers, it is
suggested that the soybean plants may be GTS 40-3-2
. No fragment was amplified when
the DNA of US or Non-GM soybeans were used as templ
ate in the PCR reaction. This is
the first report that shows GM soybeans imported to
Iran without use of the "GMO"
label in the shipment's documentation.
Keywords:
EPSPS
gene, Genetically Modified Organisms (GMOs), Monit
oring of GMOs,
Roundup ready soybean, Simplex and multiplex PCR.
