Molecular Identification and Characterization of Ph osphate Solubilizing Pseudomonas sp. Isolated from Rhizosphere of Mash Bean ( Vigna Mungo L . ) for Growth Promotion in Wheat

Abstract

Bio-inoculants have potential role in plant growth promotion. The present study evaluated the potential of Pseudomonas strains as bio-inoculants in wheat on the basis of plant growth promotion and physiological characteri zation. The 16S rRNA gene sequencing and phylogenetic analysis revealed that four isolated strains belonged to genus Pseudomonas . These strains were positive for phosphorus solubi lization and indole acetic acid production, whereas only two strains were posi tive candidate for their nitrogen fixing ability as determined by presence or absence of nifH gene through amplification from polymerase chain reaction. The pot experiment showe d that the integrated use of Pseudomonas strains as co-inoculant and 50% applied mineral fe rtilizers enhanced the maximum wheat growth and development from 58 to 140 % for different shoot and root growth parameters. The strain NCCP-45 and NCCP-237 were closely related to Pseudomonas beteli and Pseudomonas lini, respectively. These isolated strains can be used to increase crop productivity by using as a bio-fer tilizer inoculum. Keywords: Bio-inoculant, PCR, PGPR, Phylogenetic analysis, 16 S rRNA gene.