Abstract:
The objective of this study was to detect the level
of SNP variations of
rbcL
gene
sequences among and within
Prunus
species including 17 locally cultivated and wild
relatives of
Prunus,
and two species of the subfamily
Maloideae
(
Malus domestica
and
Pyrus communis
)
,
as out groups. The
rbc
L sequences were amplified, sequenced, and
aligned to determine Single Nucleotide Polymorphism
s (SNPs). The
rbcL
gene tree of the
samples showed two main clusters. The first include
d the outgroup taxa (
M. domestica
and
P. communis
); and all
Prunus
samples in the second cluster including
Prunus
armeniaca
, which separated in a subcluster. Our results indi
cate that
rbcL
gene sequence
analysis provides a well-defined tool to study rela
tionships within and among
Prunus
species, and can be successfully used in constructi
ng reliable phylogenetic tree for
Prunus
accessions.
Keywords:
Genetic relationship,
Prunus
,
rbcL
, Sequencing
