Abstract:
The yellow-bud mutant hot pepper, 96-140YBM, which
exhibits a yellow leaf phenotype
in its young leaves but whose matured leaves are gr
een, was isolated from wild type 96-
140 in this study. The results of photosynthetic pi
gment determination and chloroplast
ultrastructure observation revealed that the young
mutant leaves displayed Chl a+b and
Cars content, increased Chl a/b and Car/Chl a+b rat
ios, and delayed chloroplast
development compared with the wild type leaves. Her
e, we obtained 95,714 transcripts
from cultured yellow-bud mutant yellow leaves and c
ultured wild-type seedling leaves
using the Illumina HiSeq-2000 (Illumina Inc., USA)
platform. A total of 42,384 unigenes
were identified, among which 37,949 were annotated
using gene descriptions or gene
ontology terms. Based on Differentially Expressed G
enes (DEG) analysis, 1,056 of the
1,101 DEGs were annotated in the Nr database, and 3
02 unigenes were mapped to 130
pathways using the Kyoto Encyclopedia of Genes and
Genomes (KEGG) pathway
database. Finally, we found that 6 pathways were re
lated to chloroplast and chlorophyll
biogenesis.
Keywords:
Hot pepper; Chloroplast; Transcriptome; Yellow-bud
mutant; Wild type
