Abstract:
Strawberry is a valuable, nutritious, and economica
lly important fruit all over the
world including Bangladesh. Therefore, there is a d
emand to develop a suitable variety of
strawberry. For this purpose, leaf explants from
in vitro
grown strawberry plantlets were
cultured onto MS medium supplemented with different
concentrations and combinations
of 2,4-D, NAA and BA for callus induction. The most
effective combination was 2.0 mg/L
NAA with 0.5 mg/L BA. Then, the calli proliferated
in this medium were cultured in MS
medium containing different concentrations and comb
inations of BA, BA + NAA and BA
+ KIN + NAA for shoot regeneration. The best media
combination was 1.5 mg/L BA +
0.75 mg/L NAA + 0.5 mg/L KIN. The regenerated shoot
s were cultured onto MS medium
with different combinations of auxins or in MS and
½ MS medium without plant growth
regulators (PGRs). The highest rooting performance
was recorded in MS medium
without PGRs. The plantlets were then gradually acc
limated and successfully transferred
to the field for evaluation. Somaclonal variations
in different morphological characters
such as plant height, no. of leaves/plant, petiole
length, no. of stolon/plant, stolon length,
no. of nodes/stolon, canopy size, no. of clusters/p
lant, fruit shape, no. of fruits/plant,
average fruit wt. (g), fruit wt/plant (g), were not
iced. Some of the somaclones exhibited
better performances of the above mentioned characte
ristics than those of
micropropagated mother plants and were well adapted
to Bangladesh agro-climatic
condition and were cultivated commercially in the w
inter season by many farmers.
Keywords:
Acclimatization, Callus, Micropropagation, Plantle
ts , Regeneration.
