Abstract:
Genetic diversity in cluster bean (
Cyamopsis tetragonoloba
L.;
Fabaceae
) genotypes was
studied using Inter-Simple Sequence Repeat (ISSR) a
nd Random Amplified Polymorphic
DNA (RAPD) to derive conclusions about diversity an
alysis in groups of accessions of a
germplasm. The two methods, individually as well as
cumulatively revealed the range of
diversity in profiles among 104 genotypes collected
from different geographical regions of
India. A simulated clustering of the collected plan
t genotypes was divided into defined
affinity groups using Structure program and the clu
ster analysis of molecular markers
data revealed six broad sub-clusters. These results
were validated with a Principal
Coordinate analysis. The combined data was more inf
ormative than either of the
individual method data. The diversity range was fou
nd to be wide and the presence of six
broad clusters suggests the existence of many genet
ic lineages that can constitute useful
starting points for the use of germplasm diversity
in the selection and improvement of the
cluster bean crop.
Keywords:
Diversity, ISSR, NJ, PCA, RAPD
