Abstract:
The effects of different storage temperatures and t
imes on germination rate,
MalonDiAldehyde (MDA), SuperOxide Dismutase (SOD),
PerOxiDase (POD) and
Catalase (CAT) activities in
Jatropha curcas
L. seed were investigated. The results showed
that germination rates of seeds stored at room temp
erature and 4°C for 1 month was
significantly higher than those stored for 3 months
at -20 and -80°C. MDA contents
increased significantly for 1 and 3 months storage
with decreasing temperatures. SOD
activity decreased gradually down to -80°C for 1 and
3 months storage. POD activity
fluctuated slightly for 1 month, but increased appa
rently for 3 months storage with
decreasing temperatures. CAT activity declined rapi
dly as storage temperatures
decreased, particularly for 3 months storage. A sig
nificant interaction of storage
temperatures and times was found for the activity o
f SOD and POD, and there was no
significant interaction on germination rate, MDA co
ntent, and CAT activity.
Electrophoretic analysis showed that the observed c
hanges of SOD, POD and CAT
isoenzyme bands under different storage temperature
s were consistent with the changes
of enzyme activities assayed in extract solutions.
These results suggested that SOD, POD,
and CAT may be involved in regulating the level of
reactive oxygen species under
different storage temperatures and times.
Keywords:
Antioxidant enzyme, Germination rate, Isoenzyme pa
ttern, Malondialdehyde.
