Abstract:
The present study was carried out with the objectiv
e of validating linked SSR markers
to
Rf
genes and adopting Marker Assisted Selection (MAS)
for restorer/non-restorer line
detection in Wild Abortive (WA) type of Cytoplasmic
Male Sterility (CMS). Twelve SSR
markers reported to be linked to
Rf
genes were analyzed in the mapping population of
NedaA/Pajouhesh. Among these, three markers, namely
, RM258, RM171, and RM3148
proved to be associated with
Rf
genes. In this study, on a set of rice lines inclu
ding 2
restorers, 4 maintainers, and 9 conventional variet
ies (totally 15 genotypes of rice), MAS
with RM258 and RM171, a major
Rf
locus on chromosome 10, and RM3148, another
Rf
locus on chromosome 1, both of the
Rf
alleles in Hashemi and Deylamani varieties were
amplified the same as restorer lines. However, Pouy
a, Khazar, and Shastak had one
Rf
locus (partial restorer). Cultivars Shiroudi, Tabes
h, Fajr, and Shafaq were identified as
non-restorer (maintainer) lines. Results demonstrat
ed that these markers could be used
for screening of genotypes to identify restorers an
d non-restorer lines in hybrid rice
breeding programs.
Keywords:
Maintainer, Marker assisted selection, Restorer, R
ice, WA-CMS.
