Abstract:
Enhancement of antioxidant capacity of plants by natural antioxidants obtained from
medicinal plants can be a simple way to produce stress tolerant plants.
Zataria multiflora
essential Oil (ZO)
is one of the useful antioxidants with potent antioxidant act
ivity. ZO is
insoluble in water and can be degraded by light, oxygen, and high temperature.
Incorporation of ZO into
Poly Vinyl Alcohol (PVA)
dispersion is a simple way to improve
its stability. In this research, the antioxidant activity of PVA/ZO dispersi
on was measured
by studying the effect of the Hydrogen peroxide (H
2
O
2
) and Hydroxyl radical (HO
•
)
scavenging of the dispersion. The efficiency of the PVA/ZO dispersion on the production
of Nicotinamide adenine dinucleotide phosphate Oxidase (NOX), SuperOxi
de Dismutase
(SOD), and Catalase (CAT) mRNAs in the wheat seedlings in hydroponic condition was
investigated too.
PVA/ZO
dispersion
had a non
-
Newtonian shear
-
thinning liquid behavior
with the negative zeta
-
potential (
-
12 mV) and nanoscale particle size (13
4 nm).
PVA/ZO
dispersion had effective H
2
O
2
and HO
•
scavenging in a dose dependent manner with
IC
50
value of 220 and 170 μg mL
-
1
, respectively. PVA/ZO dispersion up
-
regulated NOX (2
folds, at 30 μg mL
-
1
), SOD (10 folds, at 30 μg mL
-
1
) and CAT (8 folds, at
250 μg mL
-
1
)
mRNAs production. Thus, there was a potent cooperation between NOX and SOD
activity and low cooperation between SOD and CAT activity. The potent antioxidant
activity of PVA/ZO dispersion implies that it can effectively be used as a promising
n
atural antioxidant to reduce oxidative stress in the plants under stress.
Keywords:
Essential oil
, CAT,
NOX,
Oxidative stress,
SOD
.
