Abstract:
Transition from vegetative to reproductive growth
is an important stage in plant’s life.
Flowering pathways including photoperiod, vernaliza
tion, gibberellins, and autonomous
pathway are regulated by different genes. Identific
ation of flowering genes is essential for
the development of bolting-resistant sugar beet cul
tivars. In this study, a set of 118
Arabidopsis
thaliana
genes involved in flowering time control were used
as a reference to
identify homologous counterparts in Expressed Seque
nce Tags (ESTs) and Transcriptome
Shotgun Assembly (TSA) sequence of sugar beet. Base
d on obtained ESTs, primers were
designed for Suppressor of Frigida 4 (
SUF4)
, Curly Leaf (CLF), Constitutive
Photomorphogenesis1 (COP1), and Cycling Dof
Factor (CDF) genes.
SUF4
and
CLF
are
components of vernalization pathway and
COP1
and
CDF
are in photoperiod pathway.
The sequence regions of these genes were amplified
using cDNA PCR technique, and
compared with other identified sequences in Gene Ba
nk. Four genes namely
CLF, COP1,
CDF
and
SUF4
were deposited in Gene Bank. Results showed that m
ost of the flowering
pathway genes in
Arabidopsis
are detectable in sugar beet which can be contribu
ted to the
understanding of the genetic control of bolting res
istance.
Keywords:
Bioinformatic, Expressed sequence tags, Flowering
genes, Shotgun Assembly
sequences, Sugar beet, Transcriptom
