Abstract:
Recently, vascular wilt
disease has caused considerable damages in tomato greenhouses.
Fusarium oxysporum
is a common pathogen of tomato and produces a variety of toxins,
pigments, and phytohormones. It has caused huge
crop
damage in south of Iran. Damage
increases in relatively
warm climate.
Fusarium oxysporum
were identified from
contaminated tissues. Thirty five of
50 isolates from samples
collected from tomato
greenhouses in southeast of Iran were identified as
F. oxysporom
. Then,
F. oxysporum
f.
sp.
lycopersici (Fol)
and
F. o
xysporum
f. sp.
radicis lycopersici (Forl)
were differentiated
from each other by host range and molecular methods.
Fol
is only pathogenic on tomato
and
Forl
is pathogenic on other hosts from Cucurbitaceae family in addition to tomato.
Molecular identifica
tion of isolates was obtained by Hirano methods. DNAs of each
isolates were reproduced by using uni, sp13 and sp23 specific primers. Based on this
study, 4 isolates were identified as race1, 4 isolates as race 2, 13 isolates as race 3, and 14
isolates as
F
orl
. This is the first report of formae
speciali
s and physiological races of this
pathogen in this region. Race 3 is reported for the first time in Iran.
Keywords
: Formae speciales, PCR, Physiological races, Specific primer.
