Abstract:
Given the growing interest in the production of
new and low cost bioemulsifiers, the
rice and wheat bran and straw were investigated in this study for the production of
bioemulsifier by
Lactobacillus plantarum
subsp.
plantarum
PTCC 1896 (probiotic). The
strain produced bioemulsifier only in the rice bra
n hydrolysate medium. The
bioemulsifier amount reached around 0.7 g L
-
1
for 72 hours of fermentation. The new
biomolecule was extracted, purified, and its structural and thermal properties were
evaluated. The functional groups and the structure of the mole
cule were revealed by
GPC, FT
-
IR,
1
HNMR and
13
CNMR techniques. The bioemulsifier was a water soluble
extracellular high molecular weight (>10
7
Da)
α
-
glucan (81.74%) bound with protein
(18.18%). Thermal behavior was studied using DSC and TG analysis. Thermal analysis
showed the bioemulsifier broke down above 211.74°C, and the melting point was 182.0°C
with the enthalpy value of 101.7 J g
-
1
. These resu
lts might provide incentives for the
industrial production of the biodegradable and safe bioemulsifier introduced in this
study, which seems to offer potential applications in the food and medical industries.
Keywords:
Probiotic, Rice bran hydrolysate, Str
uctural properties, Thermal analysis.
