dc.description.abstract |
The objectives of the study were to investigate the genetic relationships of sweetpotato
[
Ipomoea batatas
(L.) Lam] genotypes, acquired from different origins and to evaluate the
genetic relations among them,using eight SSR markers. A high level of polymorphism was
found, with an average of 7.5 alleles per SSR locus. High average values of Shannon’s
informatio
n index (0.864) and expected heterozygosity (0.739) revealed high level of
genetic diversity of the Sweetpotato genotypes. Favorable applicability and informativity
of selected set of SSR markers was confirmed by high global polymorphic information
content
(0.690) and low probability of genotype identity (
1.4×10
-
8
). The overall fixation
index was negative (
-
0.562), reflecting excess of heterozygosity, due to negative assortative
selection as a consequence of vegetative propagation of sweetpotato. Estimation
of
Rst
based on AMOVA shows 23% of molecular variance; the first two coordinates of PCoA
cumulatively explaining 62.33% of genetic variability. The assignment of individual
genotypes into three genetic groups is highly concordant with the PCoA and Bayesia
n
approach in Structure analysis. Our results suggest that selection and breeding can also
improve genetic potential and increase genetic uniformity in sweetpotato. Evaluation of
genetic background and relationships among and within genotypes provided base
line data
for introduction, management, production and conservation of sweetpotato germplasm,
regarding its favorable consumer acceptance in Central Europe.
Keywords:
Genetic structure, Genotypes, Polymorphism, SSR markers, Sweetpotato. |
en_US |