Abstract:
Saffron (
Crocus sativus
L.) is a sterile species and
biotechnological method is suggested
to improve different characteristic in this valuable plant. In this study, an efficient
protocol was provided for callus induction and regeneration of saffron using thin cell
layer explants.
Longitudinally and transvers
ally, thin cell layer explants with
approximately 1 mm thickness of apical buds were cultured on MS medium
supplemented with different concentrations of 2,4
-
Dichlorophenoxyacetic acid (2,4
-
D), 6
-
BenzylAminoPurine (BAP) and 1
-
NaphthaleneAcetic Acid (NAA). T
he highest amount of
callus induction (100%) was obtained from transverse thin cell layer explants of apical
bud in MS medium containing 2 mg L
-
1
BAP and 2 mg L
-
1
NAA during 3 months
incubation under dark condition at 20°C. The maximum percent of shoot reg
eneration
(75%) was observed on the MS medium containing 0.5 mg L
-
1
BAP. The results of this
investigation revealed that the thin cell layers from buds are suitable explants for
regeneration.
Keywords:
Callus induction,
Tissue culture, Growth Regulators,
S
hoot induction
.
