MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISATION OF BACTERIAL ROOT ENDOPHYTIC ISOLATES WITH POTENTIAL TO ENHANCE PLANT GROWTH FROM KENYAN (MWEA) BASMATI RICE

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dc.contributor.author Mbai, F. N.
dc.contributor.author et al.
dc.date.accessioned 2017-05-19T14:04:02Z
dc.date.available 2017-05-19T14:04:02Z
dc.date.issued 2017-05-19
dc.identifier.isbn 9966 923 28
dc.identifier.uri http://journals.jkuat.ac.ke/index.php/jscp/article/view/886
dc.identifier.uri http://hdl.handle.net/123456789/3117
dc.description.abstract Rice is an important grain food crop being a staple food for a large part of the world's human population. Anendophyte is an endosymbiont, often a bacterium or fungus, that lives within a plant for at least part of its lifewithout causing apparent disease. In this study, bioprospecting for bacterial rice root endophytes was explored. 66bacterial pure isolates were isolated from the 138 root samples collected from farmers’ and research fields. Theywere morphologically characterized and screened for biological activities. All isolates were gram negative rods.Two phosphates were used for phosphate solubilisation, in which case more positive response was observed incalcium phosphate than in Alluminium phosphate. Nine isolates were identified to produce IAA- indoleacetic acidimplying potential to enhance plant growth. All isolates tested negative for denitrification test. The biochemicaltests carried out helped reduce the number of isolates from 66 to 30 upon similarity and uniqueness. All 30selected isolates were confirmed to have potential to fix nitrogen based on acetylene reduction assay (ARA). Theselected 30 isolates have demonstrated to have the potential to promote plant growth in at least one biologicalmechanism. This is the first study on the endophytic nature of Kenyan (Mwea) Basmati rice endophytes. Thesemicroorganisms may be useful for agriculture (biofertilizer) to enhance affordable production for Kenyan basmatirice farmers, and also used in other applications. This will contribute to increased yield and trade of Kenyanbasmati rice to eradicate poverty and increase food security, and help conserve microbial diversity. en_US
dc.description.sponsorship JKUAT en_US
dc.language.iso en en_US
dc.publisher JKUAT en_US
dc.relation.ispartofseries Proceedings of the 2011 JKUAT Scientific, Technological and Industrialization Conference;2011
dc.subject Bacterial en_US
dc.subject biofertilisers en_US
dc.subject endophyte en_US
dc.subject isolation en_US
dc.subject isolates en_US
dc.subject rice en_US
dc.subject JKUAT en_US
dc.subject Kenya en_US
dc.title MORPHOLOGICAL AND BIOCHEMICAL CHARACTERISATION OF BACTERIAL ROOT ENDOPHYTIC ISOLATES WITH POTENTIAL TO ENHANCE PLANT GROWTH FROM KENYAN (MWEA) BASMATI RICE en_US
dc.type Article en_US


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