MICROPROPAGATION OF STRYCHNOS HENINGSII FOR SUSTAINABLE CONSERVATION

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dc.contributor.author Ngenoh, R. K.
dc.contributor.author Njenga, P. K.
dc.contributor.author Ngumi, V. W.
dc.contributor.author Onguso, J.
dc.contributor.author Kahia, J.
dc.date.accessioned 2017-05-19T12:12:53Z
dc.date.available 2017-05-19T12:12:53Z
dc.date.issued 2017-05-19
dc.identifier.isbn 9966 923 28
dc.identifier.uri http://journals.jkuat.ac.ke/index.php/jscp/article/view/891
dc.identifier.uri http://hdl.handle.net/123456789/3110
dc.description.abstract Strychnos henningsii is an important medicinal herb of the family Logoniaceae common name Muteta (Kikuyu and Kamba), Muchimbi (Meru). It is an endangered plant that is facing threat of extinction owing to indiscriminate and unsustainable harvesting in the wild. Conventional breeding is difficult in this plant, and in-vitro multiplication is important to conservation and propagation. Tissue culture techniques are useful for ex-situ conservation of rare endemic or threatened plant species. An efficient protocol for rapid production of micro shoots was established. Multiple shoots were obtained using nodal segments with two auxiliary buds as explants on Ms medium supplemented with various concentrations of Cytokinins (Kinetin, Benzyl Amino Purine) and/or GA3, at concentrations ranging from 0.5 to 10 mg/l. High frequency bud break 74.67% and multiple shoot formation were induced from nodal segments cultured on MS medium supplemented with 2.0mg/l BAP. Faster bud break coupled with enhanced frequency of shoot development 87.53% and internode elongation were dependent on the synergistic effect of GA3 (0.2 mg/l). en_US
dc.description.sponsorship JKUAT en_US
dc.language.iso en en_US
dc.publisher JKUAT en_US
dc.relation.ispartofseries Proceedings of the 2011 JKUAT Scientific, Technological and Industrialization Conference;2011
dc.subject Cytokinins en_US
dc.subject Ex-situ en_US
dc.subject medicinal plant en_US
dc.subject multiple shoots en_US
dc.subject In-vitro en_US
dc.subject Strychnos henningsii en_US
dc.subject JKUAT en_US
dc.subject Kenya en_US
dc.title MICROPROPAGATION OF STRYCHNOS HENINGSII FOR SUSTAINABLE CONSERVATION en_US
dc.type Article en_US


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