ANTIBIOTIC RESISTANCE OF METAL TOLERANT BACTERIA ISOLATED FROM SOIL IN JUJA, KENYA

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dc.contributor.author Budambula, N. L. M.
dc.contributor.author Kinyua, D. M.
dc.date.accessioned 2017-04-19T10:45:06Z
dc.date.available 2017-04-19T10:45:06Z
dc.date.issued 2017-04-19
dc.identifier.issn 9966 923 28
dc.identifier.uri http://journals.jkuat.ac.ke/index.php/jscp/index
dc.identifier.uri http://hdl.handle.net/123456789/2905
dc.description.abstract Environmental pollution and increased levels of metal concentrations may influence the composition of bacterial populations. This study aimed to determine the relationship between antibiotic resistance and metal tolerance of bacteria isolated from five soils samples collected from Juja. Bacteria were isolated by culturing in metal enriched nutrient broth and a total of 41 isolates obtained. The isolates were identified by morphology and biochemical tests. Antimicrobial resistance patterns were determined using single disc diffusion method. Tolerance to heavy metal was determined by culturing bacteria in nutrient broth containing varying concentrations of Zinc, Mercury and Cadmium. The isolates showed 100% resistance to Augmentin and Cefuroxime (n=41), followed by Ampicillin 92.68% and Cotrimoxazole 68.29%. Sensitivity to Gentamicin was 95.12%, Norfloxacin 90.24%, and Nalidixic acid 68.3%. All the isolates where multi drug resistant (MDR) with 16 (39.02%) being resistant to four antibiotics. Many of the isolates were Zinc resistant (final concentration of 7.5g/l) but sensitive to mercury (50mg/l) and Cadmium (2.8g/l). Tolerance was highest in P. aeruginosa for Cadmium at 2.0g/l, E. coli for Zinc at 5.54g/l and E. coli and S. aureus for Mercury at 40mg/l. A direct correlation was established with correlation co-efficient (r) for Cadmium +0.97, and Mercury + 0.95 and Zinc + 0.91. This study demonstrated a correlation between metal tolerance and antibiotic resistance in soil bacteria from Juja. Association of metal tolerance and antibiotic resistance is of medical concern as resistance could be transferred to pathogenic bacteria. en_US
dc.language.iso en en_US
dc.publisher JKUAT en_US
dc.relation.ispartofseries Scientific Conference Proceedings;2013
dc.subject Metal en_US
dc.subject metal tolerance en_US
dc.subject antibiotic resistance en_US
dc.title ANTIBIOTIC RESISTANCE OF METAL TOLERANT BACTERIA ISOLATED FROM SOIL IN JUJA, KENYA en_US
dc.type Article en_US


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