Broad spectrum β-lactam resistance in faecal Escherichia coli isolated from severely malnourished and nourished children attending Mbagathi district hospital, Nairobi: A case-control study

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dc.contributor.author Njoroge, Samuel Mwangi
dc.contributor.author Kiiru, John Ndemi
dc.contributor.author Kikuvi, Gideon Mutie
dc.date.accessioned 2017-03-29T07:24:55Z
dc.date.available 2017-03-29T07:24:55Z
dc.date.issued 2017-03-29
dc.identifier.uri http://www.cysonline.org
dc.identifier.uri http://hdl.handle.net/123456789/2799
dc.description.abstract Context: Severely malnourished children have increased risk of being put on antibiotics due to co-morbidities. Aim: The study’s objective was to characterize the Escherichia coli β-lactamase mediated resistance to the broad spectrum β-lactam antimicrobials among this population and compare them with nourished children as controls. Settings and Design: In this case-control, hospital-based setup, 109 E. coli isolates were obtained from each group, one isolate per subject. Materials and Methods: Stool or anal swabs were collected, enriched in buffered peptone water and cultured on MacConkey and eosin methylene blue agars. Biochemical test were used to identify E. coli. antibiograms to determine phenotypic resistance were determined using a panel of 14 drugs. Only the isolates showing synergy between ampicillin-calvulanic acid and one or more third generation cephalosporins were picked as extended spectrum β-lactamase (ESBL) producers. Statistical Analysis: Differences in ESBL rates and susceptibility percentages between cases and controls were evaluated for significance using 2-tailed Fisher’s exact test. Results: Prevalence of ESBL phenotype was higher in severely malnourished children (39%) as compared to the controls (7%). The plasmid–encoded AmpC’s (pAmpC)-like phenotype was observed in 11% isolates. Conclusions: Isolation of ESBL-E. coli among severely malnourished children is high. Surveillance of ESBL producers, both in the community and hospital settings needs to be stepped up in Kenya. en_US
dc.language.iso en en_US
dc.subject Antimicrobial resistance en_US
dc.subject diarrhea en_US
dc.subject Escherichia coli en_US
dc.subject extended spectrum β-lactamase-Escherichia coli en_US
dc.subject severely malnourished children en_US
dc.subject KENYA en_US
dc.title Broad spectrum β-lactam resistance in faecal Escherichia coli isolated from severely malnourished and nourished children attending Mbagathi district hospital, Nairobi: A case-control study en_US
dc.type Article en_US


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