Abstract:
Antimicrobial drug resistance is a rising concern in the treatment of infectious diseases
and necessitates the need for discovery of novel, potent antimicrobial compounds to
combat antibiotic resistance. Since natural environment remains a potential source of
novel antimicrobial products, this preliminary study was performed to test the potential
of soils from Kericho County for antibiotic-producing Actinomycetes. Soil samples
(214) were randomly collected from virgin soils of Kipkelion East, Kipkelion West,
Belgut, Ainamoi, Sigowet and Bureti sub-counties in Kericho County from a depth of
between 11 cm - 16 cm from the surface of the soil profile. A total of 107 Actinomycetes
were isolated and screening was done using modified agar disc diffusion method
of which only 39 (36.4%) showed antimicrobial activity against five of the six test isolates
that included reference strains Staphylococcus aureus (ATCC 25923), Escherichia
coli (ATCC 25922) and Candida albicans (ATCC 90028) and three clinical strains Trichophyton
mentagrophyte, Microsporum gypseum and Methicillin Resistant Staphylococcus
aureus. Two of the isolates showed activity against MRSA and four isolates
showed a higher potency than the standard drug Chloramphenicol (30 μg) against S.
aureus. Most of the isolates (41.0%) also showed good antimicrobial activity against T.
mentagrophyte, though they lower than the control drug Itraconazole (2 μg/ml), they
were statistically significant. DNA from the isolates was extracted and the 16S rRNA
gene was amplified using primers specific for Actinomycetes. The amplified gene was
sequenced and phylogeny analysis was done. The 16S rRNA gene was able to be amplified
in only 15 of these isolates. Sequencing showed that 93.3% were of the genus
Streptomyces while 6.7% were of the genus Rhodococcus. From the results, the soils
from this region harbour Actinomycetes that may have good potential of producing
novel antibiotics against gram positive bacteria and dermatophytes.
