Loop Mediated Isothermal Amplification for Detection of Trypanosoma brucei gambiense in Urine and Saliva Samples in Nonhuman Primate Model

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dc.contributor.author Ngotho, Maina
dc.contributor.author Kagira, JohnMaina
dc.contributor.author Gachie, Beatrice Muthoni
dc.date.accessioned 2017-02-27T12:13:08Z
dc.date.available 2017-02-27T12:13:08Z
dc.date.issued 2017-02-27
dc.identifier.uri http://dx.doi.org/10.1155/2015/867846
dc.identifier.uri http://hdl.handle.net/123456789/2719
dc.description.abstract Human African trypanosomiasis (HAT) is a vector-borne parasitic zoonotic disease. The disease caused by Trypanosoma brucei gambiense is themost prevalent in Africa. Early diagnosis is hampered by lack of sensitive diagnostic techniques. This study explored the potential of loop mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) in the detection of T. b. gambiense infection in a vervet monkey HAT model. Six vervet monkeys were experimentally infected with T. b. gambiense IL3253 andmonitored for 180 days after infection. Parasitaemia was scored daily. Blood, cerebrospinal fluid (CSF), saliva, and urine samples were collected weekly. PCR and LAMP were performed on serum, CSF, saliva, and urine samples. The detection by LAMP was significantly higher than that of parasitological methods and PCR in all the samples. The performance of LAMP varied between the samples and was better in serum followed by saliva and then urine samples. In the saliva samples, LAMP had 100% detection between 21 and 77 dpi, whereas in urine the detection it was slightly lower, but there was over 80% detection between 28 and 91 dpi. However, LAMP could not detect trypanosomes in either saliva or urine after 140 and 126 dpi, respectively. The findings of this study emphasize the importance of LAMP in diagnosis ofHAT using saliva and urine samples. en_US
dc.language.iso en en_US
dc.subject Isothermal Amplification en_US
dc.subject Trypanosoma brucei en_US
dc.subject Urine en_US
dc.subject Saliva en_US
dc.subject Primate Model en_US
dc.title Loop Mediated Isothermal Amplification for Detection of Trypanosoma brucei gambiense in Urine and Saliva Samples in Nonhuman Primate Model en_US
dc.type Article en_US


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