dc.contributor.author |
Kuria, P |
|
dc.contributor.author |
Demo, , P |
|
dc.contributor.author |
Nyende, A. B |
|
dc.contributor.author |
Kahangi, , E. M |
|
dc.date.accessioned |
2017-02-15T09:11:51Z |
|
dc.date.available |
2017-02-15T09:11:51Z |
|
dc.date.issued |
2017-02-15 |
|
dc.identifier.issn |
1684–5315 |
|
dc.identifier.uri |
http://www.academicjournals.org/AJB |
|
dc.identifier.uri |
http://hdl.handle.net/123456789/2694 |
|
dc.description.abstract |
The potential of cassava starch as an alternative and cheap gelling agent for potato in vitro culture
micro-propagation media was investigated. A two-factor experiment in randomized complete block
design was conducted. Four levels of gelling agents; 10% (w/v) cassava starch, 8% cassava starch
mixed with 0.25% agar, 0.8% agar and a liquid medium, were evaluated using three selected Kenyan
potato cultivars (Tigoni, Asante and Kenya Sifa). Cassava starch at 10% gave adequate support of
explants, though it had low viscosity and softened at 42 days after explant inoculation. Cassava starch
mixed with 0.25% agar provided the same firmness as 0.8% agar and maintained gel integrity
throughout the culturing period of 84 days. Survival in- and ex vitro was lowest in liquid medium
culture. Potato transplants from the liquid medium and cassava starch gelled medium had similar (p >
0.05) mean number of nodes and biomass. These mean values were significantly higher compared to
the transplants from the agar gelled medium. The use of 10% cassava starch reduced cost by 42.5% in
comparison with use of agar. |
en_US |
dc.language.iso |
en |
en_US |
dc.relation.ispartofseries |
African Journal of Biotechnology;Vol. 7 |
|
dc.subject |
Cassava starch |
en_US |
dc.subject |
, gelling agents, |
en_US |
dc.subject |
micro-propagation |
en_US |
dc.subject |
, transplants, |
en_US |
dc.subject |
potato. |
en_US |
dc.title |
Cassava starch as an alternative cheap gelling agent for the in vitro micro-propagation of potato (Solanum tuberosum L.) |
en_US |
dc.type |
Article |
en_US |