Abstract:
The calibration performance on sugar concentration in aqueous is shown to shift
when absorbance is collected over a range of temperature relative to calibration
developed at a single temperature. The aqueous solutions of sugar were prepared
using high purity, laboratory chemicals (HPLC) reagent sucrose in grade-3 de-ionized
water. The variation of absorbance against sugar concentration monitored at varied
temperature was found to have positive nonlinear correlation. The temperature of
sugar solution correlated well with absorbance features with PLS, R2 found to be
0.810, 0.971, 0.973 and 0.980 for 5%, 15%, 25% and 40% concentrations
respectively. This occurred due to the effect of temperature on mean kinetic energy
and mean square displacement of sucrose particulates in the ambience.
