A RAPID METHOD BASED ON UV SPECTROPHOTOMETRY FOR QUANTITATIVE DETERMINATION OF ALLICIN IN AQUEOUS GARLIC EXTRACTS

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dc.contributor.author Wanyika, H. N.
dc.contributor.author Gachanja, A. N.
dc.contributor.author Kenji, G. M.
dc.contributor.author Keriko, J. M.
dc.contributor.author Mwangi, A. N.
dc.date.accessioned 2016-10-04T12:53:48Z
dc.date.available 2016-10-04T12:53:48Z
dc.date.issued 2016-09-04
dc.identifier.citation Journal of Agriculture, Science and Technology, 2010. en_US
dc.identifier.uri http://journals.jkuat.ac.ke/index.php/jagst/search/titles
dc.identifier.uri http://hdl.handle.net/123456789/2301
dc.description.abstract Garlic (Allium sativum) is known for its medicinal and health food use. However, garlic extracts are also used in bio-pesticides. This study reports research on the optimisation of a fast and cheap method for assaying allicin, the active constituent of garlic extract, based on UV spectrophotometry. Garlic cloves were extracted using water. The allicin content of the garlic extracts was analysed after passing the extract through SPE cartridge and eluted with solvents of various polarities. The most polar solvent used was water which eluted the allicin in 4 ml, while methanol and ethanol did not. The absorbance of UV radiation at 240 nm and 254 nm wavelengths by the garlic fraction eluted with water gave a ratio of A240 nm/ A254 nm H” 1.4 – 1.5, which is typical for allicin.The garlic available in the Kenyan market was assayed for allicin using the optimised method. Some pesticides in the market containing garlic were also assayed for allicin. The results obtained compared well with documented values. en_US
dc.language.iso en en_US
dc.publisher JKUAT en_US
dc.relation.ispartofseries Journal of Agriculture, Science and Technology;Vol 12. (1)
dc.subject UV spectrophotometry en_US
dc.subject high performance liquid chromatography en_US
dc.subject garlic cloves en_US
dc.title A RAPID METHOD BASED ON UV SPECTROPHOTOMETRY FOR QUANTITATIVE DETERMINATION OF ALLICIN IN AQUEOUS GARLIC EXTRACTS en_US
dc.type Article en_US


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